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<th align="right" valign="baseline" nowrap="nowrap">Asunto:</th>
<td>Seminario/Charla del Prof Dr. Sergey Krylov</td>
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<th align="right" valign="baseline" nowrap="nowrap">Fecha:</th>
<td>2016-12-06 13:42</td>
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<th align="right" valign="baseline" nowrap="nowrap">Remitente:</th>
<td>Información Exactas <info@exactas.unlp.edu.ar></td>
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<th align="right" valign="baseline" nowrap="nowrap">Destinatario:</th>
<td>"secre, secre" <secre@mate.unlp.edu.ar>, "secre, secre" <secre@biol.unlp.edu.ar>, "secre, secre" <secre@quimica.unlp.edu.ar>, "secre, secre" <secre@fisica.unlp.edu.ar></td>
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<div class="moz-forward-container">Estimados,<br /> <br /> El próximo lunes 12 a las 14:30 en el aula del Consejo nos dará una charla el Dr. Sergey Krylov, (Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, Toronto, Canada) quien estará también unos días de visita en los laboratorios del LIDMA. <br /> <br /> El Dr. Krylov trabaja en desarrollo de métodos de análisis y de tecnologías (plataformas) aplicadas al estudio de biomoléculas, "-omicas" (genómica/ proteómica/metabolómica, etc.) y de interacciones biomoleculares. Se especializa en métodos cinéticos de electroforesis y cromatografía de líquidos, acoplados a detectores de alta "sensibilidad" (espectrometría de masas, fluorescencia, etc..). Tiene más de 150 publicaciones en revistas de máximo impacto (<a class="moz-txt-link-freetext" href="https://www.scopus.com/authid/detail.uri?authorId=7005051872">https://www.scopus.com/authid/detail.uri?authorId=7005051872</a>) y convenios con empresas como Sciex.<br /> <br /> La charla será en idioma inglés y tratará sobre las diversas líneas de desarrollo tecnológico / plataformas en las que está trabajando actualmente su grupo:<br /> <br /> <em><strong>"</strong></em><em><strong><span style="font-size: medium;">Kinetic Separation: A Conceptual Platform for Development of Homogeneous Kinetic Affinity Methods</span></strong></em><em><strong>"</strong></em><br /> <br /> <em><strong>"Separación Cinética: Plataforma Conceptual para el Desarrollo de Métodos de Afinidad Cinética Homogénea"</strong></em><br /> <br /> Se adjunta el texto del abstract de la charla. <br /> <br /> Para mayor información, contactarse con el Dr. Leonardo Gagliardi ( <a class="moz-txt-link-abbreviated" href="mailto:leogagliardi@quimica.unlp.edu.ar">leogagliardi@quimica.unlp.edu.ar</a> )<br /> <br /> -----------------------------------------------------------------------------------------------------------------------------------<br />
<p style="margin-bottom: 0cm; line-height: 100%;"><span style="font-family: 'Times New Roman', serif;"><span style="font-size: medium;"><u>Title:</u></span></span><span style="font-family: 'Times New Roman', serif;"><span style="font-size: medium;"> Kinetic Separation: A Conceptual Platform for Development of Homogeneous Kinetic Affinity Methods</span></span></p>
<p style="margin-top: 0.49cm; margin-bottom: 0.49cm; line-height: 100%;"><span style="font-family: 'Times New Roman', serif;"><span style="font-size: medium;"><u>Abstract:</u></span></span><span style="font-family: 'Times New Roman', serif;"><span style="font-size: medium;"> Non-covalent binding of biological molecules controls cellular regulation, it is also pivotal to action of modern drugs and molecular diagnostics of diseases. Therefore, understanding molecular mechanisms of biological processes as well as the development of drugs and design of diagnostic systems require affinity methods suitable for three types of applications: (i) kinetic studies of affinity interactions, (ii) selection of affinity ligands from combinatorial libraries, and (iii) the use of affinity ligands as diagnostic probes in molecular diagnostics of diseases. Our answer to this challenge is Kinetic Separation - a conceptual platform for the development of homogeneous kinetic affinity methods suitable for all three applications. One-dimensional (column) separation of any nature, e.g. electrophoresis, gel-filtration, thermophoresis, sedimentation, that can segregate affinity complexes from the unbound interactants, can be used as the basis for kinetic separation. A variety of different detection methods can be employed in kinetic separation including fluorescence and mass-spectrometry. In this lecture, I will explain the physical principles underlying kinetic separation and discuss two practical implementations of kinetic separation for (i) selection, kinetic characterization, and analytical use of DNA aptamers by means of Kinetic Capillary Electrophoresis and (ii) studying kinetics of reversible protein-drug binding by means of Kinetic Size-Exclusion Chromatography.</span></span></p>
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